Biostat® B-DCU

Overview

The Biostatae B-DCU is an industry-standard fermenter | bioreactor designed for optimization and characterization of processes in the biotechnology and biopharmaceutical industry. It provides enhanced functionality and a wide range of options for cell culture and microbial processes and is presented as an ideal scale-down model for large-scale processes. The system supports a wide range of process control strategies and offers fully flexible aeration and feeding options. Connectivity includes direct connection to BioPATae MFCS or third-party supervisory software such as DeltaV 12, and a field of connectivity is provided for sensors, balances and external pumps. The unit features a 19 " display operable with gloves and can connect UniVesselae SU 2 L, UniVesselae Glass 1 L, 2 L, 5 L & 10 L, and Sartorius Cubisae, Quintixae or Securaae balances. Aeration options include combinations of intelligent mass flow controllers with a flow range of 1: 200, gas switches and flow meters for each gas line, and the system accommodates up to four variable speed pumps and up to four fixed speed pumps. Advanced sensor and software integration is available, including combinations with BioPATae Trace, BioPATae Viamass and BioPATae Xgas and with BioPATae MFCS to enable automated and supervisory control functions. Two application notes are provided: "Precise Control of Gas Flows Within the Biostatae B-DCU" PDF | 518,0 KB | May 12, 2021 and "High Cell Density Escherichia Coli Fed-Batch Cultivation in the New Biostatae B-DCU" PDF | 804,4 KB | December 01, 2021. The Biostatae B-DCU is used for optimization and advanced characterization of bioprocesses and to mimic large-scale process control to generate representative data. For cell culture, when combined with BioPATae Trace the system can control glucose level using its local control software, perform automated glucose setpoint control, establish glucose setpoint profiles, monitor lactate concentration directly on the DCU screen and select up to four variable speed pumps for substrate addition; adding BioPATae MFCS enables advanced control loops and development of event-based glucose feeding strategies. For perfusion processes, integration with BioPATae Viamass allows operation of the Viamass probe without external software, monitoring of viable cell volume directly on the local control screen and provision of viable cell volume information to supervisory control software, while adding BioPATae MFCS enables automated feed-and-bleed perfusion control and determination of harvest or inoculum transfer timing. For microbial fermentations, integration with BioPATae Xgas supports high-accuracy gas flow control and real-time exhaust gas monitoring on the DCU, and with BioPATae MFCS provides automatic calculation of OUR, CER and RQ and a complete O2 | CO2 exhaust gas batch record.